CFX96 Touch Real-Time PCR Detection System

Description

 

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.

Key Features and Benefits
With the CFX96 Touch Real-Time PCR Detection System you can:

Set up your system quickly — easy installation and factory-calibrated optics
Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
Optimize reactions in a single run — thermal gradient feature
Analyze data faster — visualize all run data at once and export only the data you need in the format you want
Use advanced data analysis tools — normalized gene expression using CFX Maestro Software
Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
Combine the CFX96 Touch System with good laboratory practice standards — use CFX Maestro Software, Security Edition for data collection and analysis to simplify compliance with U.S. FDA 21 CFR Part 11 regulations
Flexible CFX System Configurations Meet Your Throughput Needs
The CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.

 

 

 

 

 

 

CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels
The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further

Specifications
Thermal Cycler
Chassis	C1000 Touch™
Maximum ramp rate, °C/sec	5
Average ramp rate, °C/sec	3.3
Heating and cooling method	Peltier
Lid, °C	Heats up to 105
Temperature
Range, °C	0–100
Accuracy, °C	±0.2 of programmed target at 90°C
Uniformity, °C	±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient
Operational range, °C	30–100
Programmable span, °C	1–24
Optical Detection
Excitation	6 filtered LEDs
Detection	6 filtered photodiodes
Range of excitation/emission wavelengths, nm	450–730
Sensitivity	Detects 1 copy of target sequence in human genomic DNA
Dynamic range	10 orders of magnitude
Scan Time
All channels, sec	12
FAM/SYBR® Green only, sec	3
Software
Operating systems	Windows 7, Windows 8, Windows 10
Multiplex analysis	Up to 5 targets per well
System
Licensed for real-time PCR	Yes
Sample capacity, wells	96
Sample size, µl	1–50 (10–25 recommended)
Communication interface	USB 2.0
Electrical approvals	IEC, CE
Dimensions (W x D x H)、cm/in	33 x 46 x 36 /13 x 18 x 14
Weight, kg/lb	21/47

 

Documents
NumberDescriptionOptions
A Model qPCR System Comparability Study: CFX Touch and CFX Opus Real-Time PCR Platforms	Bulletin_7444
CFX Real-Time PCR Systems Protein Thermal Shift Assay Protocol	Bulletin_7180
CFX96 Touch™ Real-Time PCR Detection System Brochure	Bulletin_7180
Tips, Tricks & Best Practices: The Ultimate qPCR Assay Design Guide	Bulletin_6894
C1000 Touch™ Thermal Cycler and CFX Real-Time PCR Detection Systems Instrument Guide, Ver A	10000068706
CFX Maestro Software Brochure	Bulletin_6900
CFX Maestro Software User Guide	10000068703_compressed
CFX96 Touch and CFX96 Touch Deep Well Real-Time PCR Detection Systems Installation Quick Guide, Ver D	10000115381
CFX Qualification Plate — 96-Well Plate Format Product Insert	10027341
Checking Firmware and Software Updates for the C1000 Touch Thermal Cycler and the CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch Real-Time PCR Detection Systems, Quick Guide, Ver D	10000115384
Plate Preparation for the CFX384™ Real-Time PCR Detection System Using a Tecan Robotics System, Rev A	Bulletin_6144
Plate Preparation for the CFX384™ Real-Time PCR Detection System Using the IDEX Health & Science Innovadyne Dispenser, Rev A	Bulletin_6149
CFX Manager™ Software, Security Edition Product Information Sheet, Rev A	Bulletin_5690
Amplification Plastics and Reagents Selection Guide	10022527
Gene Expression Analysis Solutions Brochure	Bulletin_5990
High-Throughput Real-Time PCR Solutions Brochure	Bulletin_6629
Real-Time PCR Applications Guide, Rev B	Bulletin_5279
End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR	Bulletin_7216
Optical Design of CFX96™ Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye, Rev A	Bulletin_6047A
Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing, Rev A	Bulletin_5692
Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient, Rev A	Bulletin_5962A
A Fast Semi-Automated Quantitative PCR Method for Monitoring Differential Gene Expression for Drug Target Discovery, Ver B	Bulletin_6634
Gene Expression Real-Time PCR Workflow Flier	Bulletin_6763
Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR	Bulletin_6777